Plasmid

Part:BBa_K3600017:Design

Designed by: yasmine koubaa   Group: iGEM20_EPFL   (2020-10-27)


Lys2-GLR1pr-mScarleti cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1010
    Illegal EcoRI site found at 1255
    Illegal EcoRI site found at 1835
    Illegal XbaI site found at 955
    Illegal XbaI site found at 1264
    Illegal XbaI site found at 1442
    Illegal XbaI site found at 2012
    Illegal SpeI site found at 2851
    Illegal PstI site found at 2860
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1010
    Illegal EcoRI site found at 1255
    Illegal EcoRI site found at 1835
    Illegal SpeI site found at 2851
    Illegal PstI site found at 2860
    Illegal NotI site found at 739
    Illegal NotI site found at 3415
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2582
    Illegal EcoRI site found at 1010
    Illegal EcoRI site found at 1255
    Illegal EcoRI site found at 1835
    Illegal BglII site found at 2453
    Illegal BglII site found at 2875
    Illegal BamHI site found at 2887
    Illegal XhoI site found at 2896
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1010
    Illegal EcoRI site found at 1255
    Illegal EcoRI site found at 1835
    Illegal XbaI site found at 955
    Illegal XbaI site found at 1264
    Illegal XbaI site found at 1442
    Illegal XbaI site found at 2012
    Illegal SpeI site found at 2851
    Illegal PstI site found at 2860
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1010
    Illegal EcoRI site found at 1255
    Illegal EcoRI site found at 1835
    Illegal XbaI site found at 955
    Illegal XbaI site found at 1264
    Illegal XbaI site found at 1442
    Illegal XbaI site found at 2012
    Illegal SpeI site found at 2851
    Illegal PstI site found at 2860
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 772


Design Notes

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Source

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References